Project

Part:BBa_K3982025:Design

Designed by: Akankshya Ramkrishna Sahu   Group: iGEM21_IISER_Berhampur   (2021-10-01)


CODE M Construct C1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 701
    Illegal EcoRI site found at 2153
    Illegal EcoRI site found at 2716
    Illegal XbaI site found at 616
    Illegal PstI site found at 943
    Illegal PstI site found at 1109
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 701
    Illegal EcoRI site found at 2153
    Illegal EcoRI site found at 2716
    Illegal PstI site found at 943
    Illegal PstI site found at 1109
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 701
    Illegal EcoRI site found at 2153
    Illegal EcoRI site found at 2716
    Illegal BglII site found at 710
    Illegal BamHI site found at 1747
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 701
    Illegal EcoRI site found at 2153
    Illegal EcoRI site found at 2716
    Illegal XbaI site found at 616
    Illegal PstI site found at 943
    Illegal PstI site found at 1109
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 701
    Illegal EcoRI site found at 2153
    Illegal EcoRI site found at 2716
    Illegal XbaI site found at 616
    Illegal PstI site found at 943
    Illegal PstI site found at 1109
    Illegal NgoMIV site found at 2661
    Illegal AgeI site found at 1403
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Parts from Addgene #112502 are PCR amplified and cloned in pSB1C3.

Source

Addgene #112502

References